Ab Initio Whole Cell Kinetic Model of Multi-strain Corynebacterium glutamicum (cglTY26): Research Article

Ting Yi Lim; Felice Jia Ying Ng; Nursakinah Mohamed-Khalid; Nicholas Wei Jun Liew; Farhana Abdul-Samathu; Maurice HT Ling

doi:10.58372/2835-6276.1429

Authors

Ting Yi Lim
Felice Jia Ying Ng
Nursakinah Mohamed-Khalid
Nicholas Wei Jun Liew
Farhana Abdul-Samathu
Maurice HT Ling

DOI:

https://doi.org/10.58372/2835-6276.1429

Keywords:

Whole-cell model, Kinetic model, Differential equations, Multi-strain model, Advance Syn Toolkit

Abstract

Corynebacterium glutamicum is an industrially important bacterium widely used in amino acid production. In metabolic engineering, metabolic models such as constraint-based genome scale models (GSMs) and kinetic models (KMs) can be useful computational tool guide the rational design of genetic modifications to optimize production of desired compounds in the microbe. Compared to GSMs which emphasis on steady-state flux distribution, KMs enable a comprehensive dynamic visualization of the metabolic network, by simulating the rate of change of metabolite concentration. A recent whole cell kinetic model of Corynebacterium glutamicum ATCC 13032, cglPS26, has been published. Hence, this study aims to expand cglPS26 into a multi-strain model, cglTY26, contained 1109 metabolites, 511 enzymes and their corresponding transcription and translation processes, and 1195 enzymatic reactions. This model provides a useful toolkit for further refinement, experimental validation, and future applications in metabolic engineering.

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